BACKGROUND AND OBJECTIVE: ANTIBODY SCREENING TEST IS USED IN BLOOD BANK LABORATORY AS A COMMON TEST. THIS EXAM IS USUALLY RECOMMENDED FOR PATIENTS WITH UNWANTED ANTIBODIES OR WOMEN WITH THE HISTORY OF PREGNANCY. ANTIBODY SCREENING CELLS ARE REQUIRED FOR THIS TEST AND BECAUSE OF LIMITATION IN SCREENING CELLS, WE SUGGEST A NEW METHOD, WHICH DOES NOT NEED SCREEN CELLS.

BACKGROUND: THE DEVELOPMENT OF HEMOLYTIC ALLOANTIBODIES AND ERYTHROCYTE AUTOANTIBODIES COMPLICATES TRANSFUSION THERAPY IN THALASSEMIA PATIENTS.METHODS: THE FREQUENCY CAUSES AND PREVENTION OF THIS PHENOMENON IN 90 TRANSFUSED THALASSEMIA PATIENTS WERE EVALUATED AT FATEMEH ZAHRA HOSPITAL IN BUSHEHR IN A CROSS-SECTIONAL STUDY.RESULTS: IN OUR STUDY, THE AGE OF ONSET OF SYMPTOMS RANGED FROM 40 DAYS TO 12 YEARS (1.72 ± 1.88 YEARS).

THE ADVANTAGES OF SUCH A PROGRAM ARE: - THE PATIENT SERUM IS TESTED AGAINST UNPOOLED REAGENT RED CELLS CAREFULLY SELECTED TO CARRY AS MANY ANTIGENS AS POSSIBLE D, C, E, C, E, M, N, S, S, P1, LEA, LEB, K, K, FYA, FYB, JKA AND JKB - EARLY DETECTION OF ANTIBODIES PREVENTS THE POSTPONEMENT OF SURGERY THAT MAY OCCUR WHEN ANTIBODIES ARE NOT DETECTED UNTIL COMPATIBILITY TESTS ARE DONE JUST BEFORE SURGERY...

DETECTION OF BROAD RANGE UNEXPECTED (USUALLY CLINICALLY SIGNIFICANT IMMUNOGLOBULIN AFFECTED BY IGG) AN ANTIBODY (S) AGAINST AN ANTIGEN (S) (USUALLY THE MINOR BLOOD GROUP) IN SAMPLE SERA THAT HE/SHE LACKS, IS CALLED ALLO-AB SCREENING. IT IS ALSO CALLED THE INDIRECT COOMBS TEST OR THE INDIRECT ANTI-GLOBULIN TEST (IAT). ALLO-ANTIBODY SCREENING IS KNOWN AS ONE OF THE PRE-TRANSFUSION TEST. THE PURPOSE OF PRE-TRANSFUSION COMPATIBILITY TESTING IS TO PREVENT HEMOLYTIC TRANSFUSION REACTION TECHNICAL AND CLERICAL AGAINST BLOOD COMPONENTS ALLOIMMUNIZATION WITH THE PREVALENCE RATE LESS THAN 0.5% TO UP TO 60% HAS BEEN REPORTED IN TRANSFUSED HOSPITAL-BASED PATIENTS AND IN CHRONICALLY TRANSFUSED PATIENTS INCLUDING PATIENTS AFFECTED WITH HAEMOGLOBINOPATHIES OR CANCER, RESPECTIVELY DEPENDING ON THE POPULATION AND SAMPLE SIZE OF STUDY, AND THE TEST METHOD SENSITIVITY.

INTRODUCTION: FOR THE SEROLOGICAL DIAGNOSIS OF SYSTEMIC AUTOIMMUNE RHEUMATIC DISEASES, A TWO-TIER APPROACH STARTING WITH SENSITIVE ANTINUCLEAR ANTIBODY (ANA) DETECTION BY INDIRECT IMMUNOFLUORESCENCE (IIF) ON HEP-2 CELLS FOLLOWED BY CHARACTERIZATION OF POSITIVE FINDINGS WITH DIFFERENT IMMUNOASSAYS IS RECOMMENDED. TO OVERCOME DRAWBACKS OF THIS APPROACH, WE DEVELOPED A NOVEL TECHNIQUE ALLOWING THE COMBINATION OF SCREENING AND SIMULTANEOUS CONFIRMATORY TESTING. THUS, FOR THE FIRST TIME, THIS CREATES THE BASIS FOR NEXT-GENERATION ANA TESTING...

THALASSEMIA IS AMONG THE COMMON GENETIC DISORDER WORLD WID PARTICULARLY IN THE MIDDEL EAST AND SOUTHEAST ASIA. THE OBJECTIVE OF THIS STUDY WAS TO DETERMINE FREQUENCY OF RED CELL ALLOANTIBODY AMONG B-THALASSEMIA MAJOR PATIENTS.MATERIAL AND METHOD: THE PRESENT STUDY WAS CROSS SECTIONAL INCLUDED 234 PATIENTS WITH B-THALASSEMIA MAJOR. ANTIBODY SCREENING WAS CARRIED OUT BY EMPLOYING COMMERCIAL 3 CELL PANEL USING STANDARDIZED BLOOD BANK METHODS. IF PATIENTS WERE FOUND TO HAVE AN IRREGULAR RED CELL ALLOANTIBODIES THEN THE ANTIBODY IDENTIFICATION WAS PERFORMED USING 12 PANEL CELLS. ...